癌细胞粘附分子在消化系统肿瘤骨髓转移中的作用

Detection of adhesion molecules in bone marrow metastatic tumors of digestive system.

目的探讨4种癌细胞粘附分子在不同种消化系统肿瘤骨髓转移中的作用。方法对1996—2006年中南大学湘雅医院的72例消化系统肿瘤骨髓转移病例,留取新鲜骨髓涂片,作免疫组化检测4种癌细胞粘附分子表达情况,记录其表达阳性率和积分。另有76例消化系统肿瘤无转移的患者,其中胃癌32例,肝癌20例,大肠癌24例,留取肿瘤标本作原代培养,一并作免疫组化检测。比较两组肿瘤细胞4种癌细胞粘附分子表达的差异。结果转移到骨髓的胃癌细胞与无转移胃癌细胞比较,ICAM-1、E-cad、CEA表达阳性率均明显增高(P<0.01),CD44V6表达的阳性积分显著升高(P<0.01)。转移到骨髓的肝癌细胞与无转移肝癌细胞比较,ICAM-1、CD44V6表达的阳性积分显著升高(P<0.01),E-cad两组表达阳性率与积分差别无统计学意义(P>0.05),CEA表达阳性率增高(P<0.05)。转移到骨髓的大肠癌细胞与无转移大肠癌细胞比较,ICAM-1、CD44V6、CEA表达的阳性积分显著升高,E-cad表达阳性率增高。结论4种癌细胞粘附分子的表达升高或减低在胃癌、肝癌、大肠癌骨髓转移过程中起到协同或拮抗作用。

Objective To evaluate the role of 4 types of adhesion molecules in digestive system tumors with bone marrow metastasis. Methods The fresh bone marrow slies of 72 cases of digestive system tumor with bone marrow metastasis, including 26 gastric cancer,25 liver cancer,21 colorectal cancer,were obtained. The tumor cell slides of 76 cases of digestive system tumor without metastasis, including 32 gastric cancer,20 liver cancer,24 colorectal cancer,were obtained. The expressions of 4 kinds of adhesion molecules in two groups were studied by ABC immunohistochemical methods, and positive rate was recorded and accumulated points. Results ICAM-1. E-cad and CEA positive rates/CD44 V6 accumulated points were up-regulated in gastric cancer cells in bone marrow; ICAM-1 .CD44 V6 accumulated points/CEA positive rate were up-regulated in liver cancer cells in bone marrow,while expression of E-cad had no difference;ICAM-1 .CD44V6 and CEA accumulated points/E-cad positive rate were up-regulated in large bowel cancer cells in bone marrow. Conclusion Different adhesion molecules play coordinative or resistant role in digestive system tumor with bone marrow metastasis.