反相流动注射化学发光法测定白藜芦醇

A Reverse-Phase Flow Injection-Based Chemiluminescence System for Resveratrol Determination

在氢氧化钠介质中，H2O2协同KIO4氧化鲁米诺产生强化学发光，白藜芦醇可抑制其化学发光强度，据此采用反相流动注射技术，建立了一种定白藜芦醇的化学发光新方法．在优化的实验条件下，该法测定白藜芦醇的检出限为27ng／L，线性范围为8．0×10^-8～7．0×10^-6g／L，对2．0μg／mL的白藜芦醇进行11次平行测定，其相对标准偏差为0．82％，用于实际样品中白藜芦醇的测定，结果令人满意．

A reverse-phase flow injection chemiluminescence method for resveratrol determination, which is based on the inhibition effect of the studied drug on the chemiluminescence （CL） produced from the luminol -KIO4-H2O2 system, is described in this paper. Under optimized conditions, the inhibited CL intensity on the resveratrol concentration is linear in the range 8.0 ×10^-8 - 7.0 × 10^-6 g/L with a detection limit （3a） of 27 ng/L. Relative standard deviation （RSD） is 0. 8% （n= 11） for 2 μg/L resveratrol. The proposed method has been successfully applied for resveratrol determination in synthetic samples and pharmaceutical preparations with satisfactory results.