转FRO2基因番茄突变体的抗缺铁黄化特性

Characterization of Transgenic Tomato FRO2 and Its Reaction to Iron Deficiency

为了增加植物的抗缺铁黄化能力，研究了转FRO2基因番茄突变体抗黄化特性．结果显示FRO2基因转化番茄后，使生长在缺铁胁迫下的植株根系铁还原酶活性大大增强（转FRO2基因番茄植株根系酶活性是对照2倍），番茄铁吸收能力和有效铁还原能力大大加强，植株中有效铁含量增加（转FRO2基因番茄植株叶片有效铁含量是时照的1．5倍），转FRO2基因番茄的植株叶片颜色显著比对照绿，黄化程度减轻，黄化指数降低（对照的黄化指数是转FRO2基因番茄的1．3倍），植株生长量显著高于对照（转基因植株株高高于对照50％）．在正常条件下，转FRO2基因番茄根系的FRO2基因表达量增加，铁还原酶活性增加了1．75倍，转FRO2基因番茄植株叶片有效铁含量是对照植株的2倍，是其他耐贮藏转基因番茄的（转反义NR、ACC、CTR1基因）2～3．5倍以上．

In order to increase ferric chelate in tomato plants and increase their capacity of resistance to chlorosis in salt and alkali soils, transgenic FRO2 tomato, or TFRO2, was researched, which encodes ferric chelate reductase. Under conditions of iron deficiency, the reductase activity of TFRO2 roots greatly increased, being 2 times as much as in CK. The content of ferric chelate in leaves of TFRO2 increased h3; 50% as compared with the wild plants. The leaves of TRRO2 was greener and its chlorosis index was 30% lower than the wild plants. TFRO2 grew better and the plant height was 50 % greater than CK. In normal condition, the gene expression of TFRO2 grew up, the reductase activity of TFRO2 roots was 1.75 times and the content of ferric chelate in leaves of TFRO2 in F1 and F2 generation was 2 times as much as the wild plants and 2-3.5 times as much as other transgenic tomato F1 （antisense ACS）, F2 （antisense CTR1）, F3 （antisense ACS-CTR1） and NR（antisense NR）.