蚕豆品种间过氧化物酶同工酶的初步研究

Preliminary Studies on Peroxidase Isozynles among Four Varieties in Vicia faba

实验采用垂直平板聚丙稀酰胺凝胶电泳方法,对来源于不同地域的四个蚕豆品种同一时期不同器官的过氧化物酶同工酶进行了初步研究,结果表明:供试蚕豆的过氧化物酶同工酶酶谱明显地分为慢带区和快带区;不同品种同一器官以及同一品种不同器官间的同工酶酶谱间都存在不同程度的差异;蚕豆品种间的同工酶酶谱能够真实地反映它们在遗传基础上的差异程度。这对于蚕豆的亲缘关系分析、蚕豆杂交育种的亲本选配、品种鉴定等理论研究、遗传育种和生产实践都有一定的参考价值。

The peroxidase Isozymes on different organs of the same stage from various region four Vicia faba varieties were analysed by using vertical slab polyacrylamide gel elec-trophoresis.The results were as follows:1). The peroxidase isozymes from four varieties in Vicia faba were obviously divided into faster-band belts and slower-band belts.2). The Isozymes of the same organ of different varieties and the isozymes of different organs of the same variety showed some disparities.3). The Isozyme patterns of four varieties in Vicia faba could factually show that their physical basis of heredity were different. It is significant in Vicia faba about analysing blood relationship, parental combination on cross-breeding,variety identification,heredity-breeding and product-praties.