薄层扫描法测定复方北芪口服液中黄芪甲苷的含量

Determination of the Content of Astragaloside in Fufangbeiq Oral Liquid by TLC

本文研究双波长薄层扫描法，λs=530nm，λg=700nm，测量复方北芪口服液中黄芪甲苷含量的测定方法。结果表明该法操作简便，结果准确，黄芪甲苷在1．01～5．05mg线性关系良好，回归方程Y=155．67＋724．58X，r=0．9990。

To establish a method for the determination of astragaloside A in fufangbeiqi Oral liquid by TLC-scanning. The silica gel G thin board was used. Developer was chloroform-methyl alcohol-water （13： 7： 2）. The bottom layer liquid was placed to stay overnight in refrigerator, spraying with 10% sulfuric-ethanol acid, at 105℃ heating to the spot to show a color clear. The plate was scanned with the condition of λs=530 nm, λg=100 nm zigzagly by means of double wave - length reflection. Linear range was 1. 01-5.05mg, r=0. 9990. The method is simple, accurate. It can be used for the quality of the product.