胡桃楸提取物对S_(180)肉瘤的抑制作用及机制

Inhibitory effects and immunoregulation of Juglans mandshurica maxim extract on S_(180) mouse sarcoma

目的:研究胡桃楸提取物(JMME)对小鼠S180肉瘤的抑制作用及其免疫调节作用。方法:对3组荷瘤鼠(每组10只)用0.023、0.045和0.090 g.kg-1.d-1JMME灌胃给药,同时设阴性对照组和阳性对照组,检测JMME对小鼠体内移植性肿瘤S180肉瘤的抑瘤率;以细胞培养观察JMME对S180细胞生长的抑制作用;MTT法观察JMME对肿瘤坏死因子(TNF)活性和白细胞介素2(IL-2)诱生水平的影响。结果:JMME具有明显的抑制肿瘤的作用,不同浓度的抑瘤率分别为32%、43%和56%,与阴性对照组比较差异有显著性(P<0.05);对体外培养的肿瘤细胞增殖抑制率可高达60.10%,与对照组比较差异有显著性(P<0.05);TNF活性及IL-2诱生水平较对照组明显增高(P<0.05);。结论:JMME具有显著抑制肿瘤的作用,其抑瘤作用机制是抑制肿瘤细胞的增殖并调节机体的免疫功能。

Objective To discuss the inhibitory effects of Juglans mandshurica maxim extract （JMME） on S180 mouse sarcoma and its immunity accommodation. Methods The mice bearing sarcoma in three groups （n= 10） were aderminastered with JMME with doses of 0. 023, 0. 045 and 0. 090 g · kg^-1 · d^-1 by gavage. Positive and negative control groups were set up. The inhibitory rate of JMME on S180 mouse sarcoma was detected. The cell culture was used to observe the inhibitory effect of JMME on the sarcoma, the influences of JMME on the tumor necrosis factor （TNF） activity and the interleukin-2 （IL-2） luring level were observed with MTT. Results JMME had inhibitory effect on the sarcoma. The inhibitory rates in JMME groups with different does were 32%, 43%, 56%, respectively, there were significant differences compared with negative group （P〈0. 05）. The inhibitory rate of JMME on tumor cell cultivated in vitro （60.10%） was higher than that in control group （P〈0.05）. The TNF activity and IL-2 luring level in JMME group were higher than those in control group （P 〈 0.05）. Conclusion JMME has significantly inhibitory effect on S180 mouse sarcoma. Its mechanism is associated with inhibition of the growth of the exosomatic tumor and regulation of immunity function of the body.