COX-2基因在人脑星形细胞肿瘤组织中表达及其与Fas基因表达的相关性

Expression of cyclooxygenase-2 mRNA in human astrocytic tumors and its correlation with Fas mRNA expression

目的:探讨COX-2 mRNA在星形细胞肿瘤组织中的表达及与Fas mRNA表达的相关性。方法:应用RT-PCR技术检测不同级别星形细胞肿瘤组织及非肿瘤组织中COX-2 mRNA和Fas mRNA的表达水平。结果:COX-2 mRNA表达阳性率分别为:非肿瘤组织33.3%,肿瘤组织78.4%;Fas mRNA阳性表达率分别为:非肿瘤组织8.3%,肿瘤组织74.5%;二者在肿瘤组织中的表达显著高于其在非肿瘤组织中的表达(P<0.05),且有良好的相关性(r=0.525,P=0.008)。结论:COX-2 mRNA表达与星形细胞肿瘤的发生、发展有关,是评估星形细胞肿瘤恶性生物学行为的可靠指标;COX-2 mRNA表达与Fas mRNA密切相关,两者可能存在共同的激活机制,构成抑制肿瘤细胞凋亡的多种途径。

Objective To detect the expression of COX-2 mRNA in astrocytic tumors （ATs） tissue, also to analyze its correlation with the expression of Fas mRNA, Methods Reverse transcription polymerase chain reaction （RT- PCR） technique was used to examine the levels of expression of COX-2 mRNA and Fas rnRNA in ATs tissue and non-tumor tissue. Results The rates of of COX-2 mRNA in non-tumor tissue and ATs tissue were 33.3%and 78. 4%, respectively. The positive exprssion rates of Fas mRNA in non-tumor tissue and ATs tissue were 8. 3% and 74.5%. The expression levels of COX-2 mRNA and Fas mRNA in ATs tissue were significantly higher than those in non-tumor tissue （P〈0.05）. There was obvious correlation between expressions of COX-2 mRNA and Fas mRNA （r=0. 525, P=0. 008）. Conclusion The expression of COX-2 mRNA is correlated with carcinogensis and development of ATs, and it is an effective marker in diagnosis of ATs. A positive correlation between expressions of COX-2 mRNA and Fas mRNA is found, it is speculated that there may be a common transcription mechanism which constructs multiple pathways contributing to the inhibition of apoptosis in ATs.