骨桥蛋白在结肠癌组织中的表达及血清水平检测

Expression of osteopontin in colon cancer tissue and detection of osteopontin in serum

目的:探讨骨桥蛋白(OPN)在结肠癌中的标志性作用及与术后复发的关系。方法:应用酶联免疫吸附试验(ELISA)检测58例结肠癌患者(观察组)及22例结肠镜检查正常者(对照组)血清中OPN水平,采用逆转录聚合酶链反应(RT-PCR)半定量法检测组织中OPN mRNA的表达,并观察术后1年复发病例(23例)和未复发病例(35例)结肠癌组织OPN mRNA表达及血清OPN水平。结果:观察组血清中OPN水平及结肠癌组织OPN mRNA表达均高于对照组(P<0.01)。结肠癌术后1年内复发者血清OPN水平及OPN mRNA的表达明显高于未复发者(P<0.01)。结论:OPN是一种肿瘤标志物,在结肠癌患者中有标志性作用。可被用于结肠癌的诊断和预后估计。

Objective To explore the effect of osteopontin （OPN） as marker in colon cancer tissue and analyze the relationship between osteopontin and recurrence. Methods ELISA method was used to determine the serum levels of osteopontin in 58 patients with colon cancer （observation group） and 22 cases of normal subjects （control group） ; semi-quantity RT-PCR was used to detect the expression of osteopontin in tissues. The expression level of OPN mRNA in colon cancer tissue and serum OPN level in 23 recurrence cases and 35 non-recurrence cases one year after operation were observed. Results The OPN mRNA expression in colon cancer tissue and the serum osteopontin level in patients in observation group were higher than those in control group （P〈0. 01）. The OPN mRNA expression and the serum level in patients with recurrence one year after operation were higher than those without recurrence （P〈0.01）. Conclusion Osteopontin is one of the tumor markers and it could be used in diagnosis and prognosis evaluation of colon cancer.