CD44基因在非小细胞肺癌组织中的表达及其与预后的关系

Expression of CD44 gene in non-small cell lung cancer tissue and relationship with prognosis

目的:探讨CD44基因在非小细胞肺癌(NSCLC)组织中的表达及其与临床预后的关系。方法:应用RT-PCR方法检测36例NSCLC组织中CD44基因的表达,并进行3年随访。结果:36例NSCLC组织有21例CD44基因过量表达,过量表达率为58.3%。CD44 mRNA在NSCLC组织中的表达与患者的年龄、性别、组织学类型、病理分级、TNM分期及肿瘤大小无关,而与淋巴结转移有关(χ2=9.787,P<0.01)。③经Kaplan-Meier生存曲线分析表明,36例NSCLC组织中CD44过量表达组生存率低于一般表达组(χ2=5.9116,P<0.05)。结论:CD44基因过量表达的NSCLC患者淋巴结转移率高,CD44基因的表达检测对NSCLC患者的预后判断和术后放疗及化疗具有指导性作用。

Objective To study the expression of CD44 gene in non-small cell lung cancer （NSCLC） tissue and the relationship with prognosis. Methods The expressions of CD44 gene in 36 specimens from 36 patients with NSCLC were determined by RT-PCR and all the patients were followed up for 3 years. Results CD44 gene was excessively expressed in 21 of 36 specimens of NSCLC tissues, excessive expression rate was 58. 3%. The expression of CD44 mR NA in NSCLC tissue was related to metastasis of lymph node （X^2 = 9. 787, P〈0.01）, not related to age, sex, histological types, pathological grades, TNM stages, tumor size of the patients. Kaplan-Meier survival curve showed that the survial rate of CD44-positive NSCLC tissue was lower than that in CD44-negative NSCLC tissue （X^2 =5. 9116, P〈0.05）. Conclusion The rate of lymph node metastasis is high in NSCLC patients with CD44 gene excessive expression. The detection of CD44 gene expression can guide the prognosis, radiotherapy and chemotherapy of patients with NSCLC after operation.