趋化因子与狼疮性肾炎和慢性肾炎的相关性研究

Correlation of chemokines with lupus nephritis and chronic glomerulonephritis

目的了解狼疮肾炎、慢性肾小球肾炎患者血、尿趋化因子含量的变化并探讨其在发病中的意义。方法用双抗体夹心ELISA方法检测20例狼疮性肾炎、20例慢性肾小球肾炎以及20例健康志愿者血清及尿液巨噬细胞炎症蛋白-1α(MIP-1α)、巨噬细胞炎症蛋白-1β(MIP-1β)、激活正常T细胞表达和分泌因子(RANTES)的含量,分析狼疮肾炎和慢性肾小球肾炎间血、尿趋化因子水平的差异。结果与正常对照组比较,血清MIP-1α含量在狼疮肾炎和慢性肾小球肾炎患者中均显著升高(P<0.001,P=0.004),血清MIP-1β含量在慢性肾小球肾炎患者中显著升高(P=0.012),尿液MIP-1β含量狼疮肾炎组显著升高;血、尿间的MIP-1α、MIP-1β、RANTES含量无显著相关。结论MIP-1α、MIP-1β可能与狼疮肾炎、慢性肾小球肾炎发病有关,不同的趋化因子在不同肾炎中的作用可能不同。

Objective To explore the concentration change and it＇ s significance of serum and urine chemokines in patients with lupus nephritis （LN） and chronic glomerulonephritis （CGN）. Methods Concentrations of MIP-1α,MIP-1β and RANTES in serum and urine were measured by double antibody sandwich enzyme - linked immunoabsorhent assay in 20 lupus nephritis patients, 20 chronic glomerulonephritis patients and 20 healthy volunteers, Difference of three chemokines between groups was analysed, Results The serum concentrations of MIP - 1α was increased significantly in LN and CGN patients （P 〈 0.001, P=0.004） , the serum concentration of MIP-1β increased significantly in CGN patients （P = 0.012 ）, the urine concentration of MIP -1β was elevated significantly in LN group ; No correlations was found between serum and urine MIP -1α, MIP -1β RANTES, Conclusion MIP - 1α and MIP -1β are perhaps involved in the pathogenesis of LN and CGN,