补充活性肽对离心运动后骨骼肌微损伤及修复效果的观察研究

Study on Intervention of Active-peptides on Micro-injury and Repair of Skeletal Muscle after Eccentric Exercise

研究目的:观察活性肽对离心运动后骨骼肌微损伤及修复的营养干预效果。研究方法:以大鼠离心运动为模型,分别观察补蛋白和补肽对运动后各相关指标的变化情况。研究结果:1)补肽比补蛋白对运动引起的骨骼肌微细结构和超微结构的异常变化具有更好的抑制作用且对其恢复具有更好的促进作用;2)补大豆蛋白和大豆活性肽都可减轻抗结蛋白抗体染色脱染和增加抗波形蛋白抗体对波形蛋白的免疫反应分值的现象,但大豆活性肽的作用效果更佳;3)运动后,血清CK活性和MDA含量升高,CK在运动后24h达峰值,48h以后逐渐开始恢复,MDA在运动后24h达峰值,72h以后才逐渐开始恢复,但CK和MDA的变化没有相关性,补肽在24h左右就能抑制运动后CK活性的升高,在48h左右就能抑制运动后MDA的升高,而补蛋白在运动后72hCK和MDA的水平还不能恢复;4)运动后,尿3-MH含量急剧上升并在即刻达峰值,48h以后开始恢复,补肽比补蛋白对运动引起的尿3-MH含量升高的抑制作用更佳,且对其运动后的恢复具有更好的促进作用;5)运动后即刻骨骼肌β-葡萄糖苷酸酶含量急剧上升并达峰值,直至运动后72h仍没能恢复,运动后骨骼肌G6PDH含量明显上升,在运动后24h达峰值,直至运动后72h仍不能恢复,甚至还稍有上升;补大豆多肽能显著降低运动引起炎症反应两种标志酶升高的作用,但补蛋白对其变化的影响不明显。结论:研究揭示了活性肽对运动性骨骼肌微损伤和修复有很好的营养干预作用,其机制主要体现在促进运动后微损伤的修复方面。

The purpose of this study was to observe the effects of nutrition intervention while supplied active-peptides. Taking eccentric exercise of rats as research model, the variables of each index were observed after exercise when supplying peptide and protein. The result shows that supply peptides have finer restrain effect again these abnormal changes and accelerate effect to repair these changes than supply protein. Supply peptides and protein can alleviate dye of anti-desmin antibody and positive dye of anti-vimentin, but the former has finer effects than the later. The level of serum CK and MDA increased after eccentric exercise and reached peak in 24 hours and down to the level of CK and MDA after 72 hours. The content of urine 3-MH sharply enhanced and reached peak in zero hour, and begin to resume in 48 hours. Supply peptides have finer restrain effect than supply protein. The content of skeletal β- GUS sharply enhanced and reached peak in zero hour, but can not resume in 72 hours. Supply peptides could obviously decline the enhancement of the two sign enzymes of inflammation reaction, but the effect of supply protein was not obvious. It concludes that supply peptides and protein can pre- vent the occurrence of exercise-induced micro-injuries of skeletal muscle and its mechanism of peptides mostly lied on accelerating repairs.