不同低氧训练方式对红细胞调控T淋巴细胞免疫及亚群的影响

The Effect of Modulation of Erythrocyte on Lymphocyte Immune Function and of Lymphocyte Subpopulations at Different Hypoxia Training

观察不同低氧训练方式对红细胞调控T淋巴细胞免疫及亚群的影响。用低氧舱模拟4000m高原低氧环境(12.6%氧浓度),将50只SD雄性大鼠随机平分为5组:高住高训组(HiHi)、高住低训组(HiLo)、高住对照组(HiCo)、低住低训组(LoLo)和低住对照组(Lo-Co)。实验4周,每周6天游泳,1天休息,每天训练1.5h。实验结束后,同时取腹主动脉血,用流式细胞仪检测红细胞CD48、CD59荧光强度和T淋巴细胞CD2+%、CD3+%、CD4+%、CD8+%。结果发现,红细胞CD48、CD59表达和T淋巴细胞CD2+%、CD3+%、CD4+%、CD8+%大致趋势是HiHi组<HiCo组<HiLo组<LoCo组<LoLo组,各组间CD4+/CD8+比值无显著性差异(P>0.05)。提示:1)低氧暴露可能会降低红细胞调控T淋巴细胞免疫能力;常氧环境中运动有可能促进红细胞调节T淋巴细胞免疫;2)低氧暴露和常氧环境中训练都有可能影响T淋巴细胞总数;3)不同低氧训练方式中CD4+/CD8+比值没有显著性差异。

To observe the effect of regulation of erythrocyte on lymphocyte immune function and of Lymphocyte subpopulations at different hypoxia training （12.60%O2 , simulated altitude of 4,000 m）. 50 male SD rats were divided into five groups randomly： HiHi, HiLo, HiCo, LoLo and LoCo groups for four weeks experiment with six days training per week, 1.5hours everyday and one day rest in a week. At the end of experiment, flow cytometry was adopted to detect the expression in CD48, CD59 on erythrocyte and CD2, CD3, CD4, CD8 on T lymphocyte. There was a trend that their expression decreased, LoLo〉LoCo〉 HiLo〉 HiCo 〉 HiHi groups,and was not significant variability of CD4^＋/CD8^＋ between different group. It is implied that the modulation of erythrocyte on T lymphocyte immune function weakened at hypoxic condition, but improved in normoxia training. Hypoxic exposure and training in normoxia could affect total lymphocyte count.