肠外营养对大鼠移植小肠萎缩及功能低下的预防作用

A Study on the Prevention of Atrophy and Hypofunction of the Transplanted Small Bowel by Parenteral Nutrition in the Rat

用改良的Monchik和Russell法对近交系Wistar鼠行近段空肠异位移植,然后给予肠外营养10天,结果:加谷氨酰胺的实验组移植小肠的绒毛高度、粘膜厚度、腺窝深度和绒毛表面积值均明显大于对照组;实验组DNA倍体分布百分率与对照组明显不同.实验组移植小肠粘膜上皮细胞的超微结构基本保持完好.动态观察移植肠对^(15)N-苷氨酸的吸收率发现,实验组在各时间点的吸收率均大于对照组.结果提示:加谷氨酰胺的肠外营养能促进移植小肠粘膜增生,维持细胞超微结构的完整,并能改善其对氨基酸的吸收能力.

Having received a heterotopic jejunal isograft according to a method modified from Monchik and Russell Wistar rats were catheterized and given parenteral nutrition for 10 days. In the group that received Glutamine-enriched solution (study group), mucosal villous height, mucosal thickness, crypt depth and villous surface area were all increased significantly as compared to the non-glutamine group (control group). There were also remarkable differences between the 2 groups in terms of their distribution (%) of various DNA-ploidy patterns as well as the absorption rate of 15N -Glycine of their grafts. Enterocyte ultrastructure was practically maintained intact in grafts of the study group. These results indicated that the glutamine-enriched solution has the advantages to promote the proliferation of the intestinal mucosa, to maintain the ultrastructure of the enterocytes, and to improve the amino acid absorption rate in the small bowel isograft.