氟苯尼考在中国对虾体内消除规律的研究

Elimination of Florfenicol in Fenneropenaeus chinensis

采用口服药饵的方法研究氟苯尼考在中国对虾体内的代谢动力学和残留规律,用反相高效液相色谱测定氟苯尼考在对虾组织中的含量。结果表明:中国对虾连续5 d口服氟苯尼考药饵,高、低2种剂量(16 mg/kg.d和8 mg/kg.d)组的药物代谢动力学变化趋势相似,高剂量组的组织中药物含量远高于低剂量组,肝脏中的药物浓度远大于肌肉中的药物浓度,氟苯尼考在对虾体内的消除速度较快,低剂量组的消除速度比高剂量组快,2种剂量的药物在对虾肌肉中的消除曲线方程分别为C(t)高=0.247 3 e-0.015 5 t和C(t)低=0.118 2 e-0.043 2 t,消除半衰期分别为44.71 h和16.04 h。在本试验条件下,高、低2种剂量的氟苯尼考在肌肉组织中降到0.01,0.05,和0.1μg/g不同浓度水平的理论时间为:207,103,58,57,20和4 h。

Pharmacokinetics and residues of florfenicol following multi-oral administration were evaluated in shrimp, Fenneropenaeus chinensis. The shrimp were treated with florfenicol at doses of 8 mg· kg^-1 and 16 mg · kg^-1 weight body per day for five days at （23±1 ） ℃. At different times after oral administration, six shrimps were randomly selected from the tank and killed. Muscle and hepatopancreas tissues of the selected shrimp were analyzed by high-performance liquid chromatography （HPLC）. The results showed that the tissue distribution and elimination profiles of florfenicol at the two doses werevery similar but the florfenicol concentration in high dose was much higher than that in low dose. The elimination rate of florfenicol in Fenneropenaeus chinensis was high, and the rate in the low dose group eliminated quicker than the high dose group. The elimination equations in the two groups were C（t）high = 0. 247 3 e^-0.015 5t and C（t）tow = 0. 118 2 e^-0.043 2t, and the elimination half-lives were 44.71 h and 16.04 h , respectively. The theoretical times were 207 h, 103 h, 58 h and 57 h, 20 h, 4 h, respectively when the florfenicol concentrations in shrimp muscle and hepatopancreas fell to 0.01, 0.05 and 0.1 μg/g.