新型螯合剂对镉致小鼠睾丸毒性的解毒作用

Effects of new chelating agents on testicular toxicity in mice induced by acute exposure to cadmium

目的探讨新型螯合剂N-对羟甲苯甲基-D-葡萄糖二硫代氨基甲酸钠(HBGD)和N-苯甲基-D-葡糖二硫代氨基甲酸钠(BGD)对镉致小鼠睾丸毒性的解毒作用。方法研究镉染毒组小鼠和HBGD、BGD、二乙基二硫代氨基甲酸钠(DDTC)、二巯基丙醇(BAL)、乙二胺四乙酸(EDTA)等5种螯合剂治疗组小鼠睾丸中镉及钙、铁、锌等必需金属浓度、小鼠睾丸脂质过氧化物(LPO)值、小鼠血清乳酸脱氢酶(LDH)活性及雄性小鼠生殖能力的变化。结果镉染毒(2.5 mg/kg,腹腔注射)后,小鼠睾丸中镉浓度显著升高,睾丸钙、铁、锌等金属浓度、睾丸LPO值及小鼠血清LDH活性显著高于对照组。分别于染镉30min和24h后腹腔注射各螯合剂(400μmol/kg)。治疗24 h后,HBGD和BGD显著抑制了上述各指标的升高(P<0.05),并对镉引起的雄性小鼠生育能力的下降有明显改善作用。结论HBGD和BGD对镉致小鼠睾丸毒性有较好的解毒作用,且自身毒性小,有望成为镉的解毒剂。

Objective To study the effects of new chelating agents N - p - hydroxy - methyl - benzyl - D - glucamine dithiocarbamate（HBGD）and N- benzyl- D- glucamine dithiocarbamate（BGD） on testicular toxicity in mice induced by acute exposure to cadmium. Methods HBGD, BGD, diethyldithiocarbamate （DDTC）, 2, 3 - dimercaptopropanol （BAL）, and ethylene diarninetetraacetic acid（EDTA） were studied for their effects on the concentrations of cadmium（Cd）, calcium（Ca）, iron（F e）, and zinc（Zn） in testes, lipid peroxidation（LPO） value of testes, activity of lacatate dehydrogenase（LDH） in serum and reproductive ability of male mice induced by acute exposure to Cd. Results Mice were injected intraperitoneally with Cd- Cl2（2.5 mgCd/kg） and after 30 min or 24 h, they were injected intraperitoneally with chelating agents（400/μmol/kg）. Cd injection increased in the concentrations of Ca, Cd, Fe, Zn, and LPO value in testes as well as the activity of LDH in serum of mice. HBGD and BGD effectively prevented the increase in above indices, and dearly improved the reproductive ability weakened by exposure to Cd. Conclusion HBGD and BGD are not only more effective detoxificationon testicular toxicity in mice induced by acute exposure to Cd, but also less toxic, so they are pretect antidote for CAl.