摘要
目的:研究黄芪、川芎两种中药对庆大霉素(GM)致大鼠急性肾功能衰竭(ARF)的预防效果。方法:将大鼠随机分为5组:Ⅰ组为正常对照组;Ⅱ组为模型组;Ⅲ组为川芎预防组;Ⅳ组为黄芪预防组;Ⅴ组为黄芪+川芎预防组。动态观察各组动物的尿量、血浆肌酐(PCr)和尿素氮(BUN)、血浆和肾皮质匀浆的超氧化物歧化酶(SOD)及丙二醛(MDA)、肾脏组织光镜形态变化。结果:实验第4d,模型组和各预防组的尿量、PCr、BUN较正常对照组有非常显著性升高(P<0.01)。实验第7d和第11d,各预防组的尿量、PCr、BUN、血浆和肾皮质匀浆的MDA和SOD较正常对照组和模型组均有极显著性差异(P<0.01)。实验第14d,各预防组的尿量、PCr、BUN、血浆和肾皮质匀浆的MDA和SOD较正常对照组无显著性差异(P>0.05),各预防组的尿量、PCr、BUN、血浆和肾皮质匀浆的MDA和SOD较模型组有显著性差异(P<0.01或P<0.05)。在第7d和第14d,正常对照组的肾组织光镜形态无异常改变。第7d,模型组的肾小管上皮细胞广泛坏死、脱落,间质水肿,大量炎性细胞浸润,各预防组以肾小管上皮细胞较广泛变性为主,有少量炎性细胞浸润,坏死少见。第14d,各预防组肾小管基本修复,而模型组的仅见部分肾小管再生修复。结论:黄芪和川芎对GM致大鼠ARF有很好的预防作用,这可能与清除氧自由基的机制有关。此项研究对临床使用黄芪、川芎预防GM致ARF提供了有意义的理论和实验依据。
Objective： To investigate the preventive effect of Chinese herbs （Astragalus, AS; Chuanxiong, CX;a composition of Astragalus and Chuanxiong, ASCX） on acute renal failure （ARF）caused by gentamicin （GM）. Methods： Male Wistar rats were divided randomly into： Ⅰcontrol group;Ⅱ model group; Ⅲ CX prevention group;Ⅳ AS prevention group;Ⅴ ASCX prevention group. The changes of urine volume, blood urea nitrogen（BUN）, plasma creatinine（PCr）, malondialdehyde（MDA）and superoxide dismutase（SOD） in plasma and renal cortex were observed serially and renal tissue was observed serially with light microscopy. Results： On the 4th day, the urine volume, PCr and BUN in model group and every prevention group were significantly higher than those in control group（P 〈 0.01）. On the 7th and l lth day, the urine volume, PCr, BUN, MDA and SOD in plasma and renal cortex in every prevention group showed significant differences with those in control group and model group（P 〈 0.01）. On the 14th day, the urine volume, PCr, BUN, MDA and SOD in plasma and renal cortex in every prevention group showed no differences with those in control group（P 〉 0.05 ）, and significant differences with those in model group（P 〈 0.01 or P 〈 0.05）. On the 7th day, light microscopy showed extensive necrosis and dropping in kidney tubule epithelial cells（KTEC） in model group, but KTEC showed extensive degeneration in every prevention group, and necrosis was seldom. On the 14th day, light microscopy showed that KTEC were almost regenerated in every prevention group and partly regenerated in model group. Conclusion：The data suggest that As and CX may play a role in the protection of ARF caused by GM as an oxygen free radical scavenger. The study provides significant basis of theory and experiment for clinical using AS and CX to prevent ARF caused by GM.
出处
《内蒙古医学杂志》
2007年第2期146-150,共5页
Inner Mongolia Medical Journal
