DNA加合物^（32）磷后标记检测中［γ─^（32）P］ATP应用剂量的研究

THE USING OF[γ ￣(32)P]ATP IN DETECTION OF DNA ADDUCTS BY ￣(32)P-POSTLABELING TECHNIQUE

本研究以不同剂量［γ─￣（３２）Ｐ］ＡＴＰ对染苯小鼠外周血白细胞ＤＮＡ加合物进行检测，结果发现２０、５０、１００、１５０μＣｉ组均可得到２种ＤＮＡ加合物，ＣＰＭ差异不大，这不仅可减少同位素用量节省费用，且可降低其对实验者的危害。

This paper reports the detection of DNA adducts in peripheral leukocvtes of mice exposed tobenzene by using different dose of[γ ￣(32)P]ATP. There are two kinds of DNA adducts in all groups(20,50,100,150μCi).No remarkable difference was found among them.