发育大豆子叶细胞中酸性磷酸酶的亚细胞学定位

ACID PHOSPHATASE LOCALIZATION IN COTYLENARY CELLS OF DEVELOPING SOYBEAN SEEDS

本实验采用电镜细胞化学技术，观察了酸性磷酸酶在发育中大豆子叶细胞内的亚细胞学分布。酸性磷酸酶除分布在细胞核内的部分染色质上，内质网膜上，质体胞质小泡和胞质中，主要积累于蛋白体内。酸性磷酸酶在不同蛋白体内分布有所不同：１）在一部分蛋白体中，酸性磷酸酶均匀散布于蛋白质基质上；２）在另一部分蛋白体内，酸性磷酸酶成簇沉积于其中；３）还有一部分蛋白体，其中央为致密区，边缘为疏松区，酶在边缘疏松区的含量明显高于中央致密区中酶的量。

The cytochemical localization of acid phosphatase wasstudied in the cotyledanary cells of developing soybean seeds. Acid phosphatase activity was observed in the following loci: part of chromatin in the nucleus, membrane of endoplasmic reticulum, plastids, vesicles, cytoplasm and mainly in protein bodies. Dense enzyme reaction product was located in proteinaceous materials of the different protein bodies in either uniform or dotted patterns. There were some kinds of protein bodies with the compact center and the sparse periphery. Dense enzyme reaction product was far more in the sparse periphery than that in the compact center.