补肾活血药对子宫内膜异位大鼠腹腔液的影响

Effect of Peritoneal Fluid of Endometriosis Model Rats to Function of Human Sperm and Chinese Herbs Invigorating Kidney to Promote Blood Circulation

目的了解不同中药对大鼠子宫内膜异位症动物模型腹腔液对精子功能的作用。方法取造模成功子宫内膜异位大鼠32只分为4组,取正常大鼠8只,分别取腹腔液,其中3组造模成功大鼠给活血化瘀、补肾活血、单纯补肾药物治疗后取腹腔液,将腹腔液与正常精液标本混合孵育后,计数其A级直线向前活动率及总活动率,置低渗液中计数其总肿胀率。结果与正常大鼠组相比,造模成功组腹腔液孵育后精子A级直线向前活动率及总活动率、总肿胀率均呈明显下降(P<0.05,P<0.01,P<0.01),经不同中药治疗后,补肾活血组上述指标基本恢复正常,与正常大鼠组相比均无显著差异(P>0.05,P>0.05,P>0.01)。结论子宫内膜异位症大鼠腹腔液对精子功能有显著影响,而补肾活血中药则有一定治疗作用。

Ibhective To analyze the effect of peritoneal fluid of endometriosis model rats to human sperm function and the treatment effect of different Chinese herbs. Methods 8 normal rats as control group. 32 endometriosis model rats randomly divided into four groups ,3 of them are treated with herbs for invigorating the kidney to promote blood circulation, promoting blood circulation to remove blood stasis, improve kidney asthenia After treatment , get the peritoneal fluid with other two groups and incubated with normal sperm for sperm test and hypo - osmotic ratio. Results The peritoneal fluid with endometriosis model rats made sperm motile ratio and general hypo - osmotic ratio significantly lower than that of control group（ P 〈0. 05, P 〈0. 01, P 〈0. 01 ） ,herbs for invigorating the kidney to promote blood circulation could restore this change（ P 〉0.05, P 〉0.05, P 〉0.01 ）. Conclusion The peritoneal fluid with endometriosis model rats have significantly effect to sperm function, herbs for invigorating the kidney to promote blood circulation could restore this change.