单克隆抗体PAP法对实验感染雏鸭体内鸭肝炎病毒的定位检测

DETERMINING THE LOCATION OF DUCK HEPATITIS VIRUS IN INOCULATED DUCKLINGS BY MONOCLONAL ANTIBODY-PAP TECHNIQUE

本实验运用单克隆抗体ＰＡＰ法，对实验感染鸭肝炎病毒雏鸭的组织切片进行染色观察，旨在动态研究病毒在雏鸭体内的分布以及病毒与组织病变的关系。研究结果显示，感染后３ｈ，雏鸭心肌、肝、脾、肾、胰、腿部肌肉、大脑等组织的细胞浆内均出现了ＰＡＰ染色的特异性棕黄色产物，除胰脏组织切片的ＰＡＰ反应性在感染后１６８ｈ达到最高外，上述其他组织的最高ＰＡＰ反应性均在感染后２４ｈ；对同期的不同组织切片比较观察发现，肝、脾、胰外分泌腺、肾小管上皮细胞及腿部肌肉细胞的ＰＡＰ反应性比心肌及大脑神经元细胞的ＰＡＰ反应性强。由此表明，鸭肝炎病毒在感染后３ｈ达全身化，并主要侵害肝、脾、肾、胰及腿部肌肉。

The sections of tissues from inoculated ducklings with duck hepatitis virus type I(DHV-Ⅰ)were stained by monoclonal antibody-PAP technique for studying dynamically the distribution of virus in the inoculated ducklings and the relationship between virus and histopathological changes.The results showed that the brown-yellow products of PAP reaction were observed in the cytoplasm of heart,liver,spleen, kidney, pancrea, brain and leg muscles of the inoculated ducklings 3 hours after inoculaton.The reactivity of PAP in pancreatic section reached the highest 168 h after inoculation, but for all other tissues, 24 h after inoculation.The comparative results showed that the reactivity of PAP was high in liver and spleen, but low in heart and brain in all sections at the same time after inoculation.These results indicated that DHV had distributed in all tissues 3 h after inoculation and invaded specially in cytoplasm of liver,spleen,pancrea, kidney and leg muscles, and consequently histopathological changes occurred.