DDPH对缺氧内皮细胞条件培养液致肺动脉平滑肌细胞PDGF　mRNA表达的影响

The Effect of DDPH on the PDGF mRNA Gene Expression of Pulmonary Artery SMC Induced by the HECCM

利用地高辛标记的ｃＤＮＡ探针，原位检测ＤＤＰＨ［１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙胺基）丙烷酸盐］对缺氧内皮细胞条件培养液致猪ＰＡＳＭＣＰＤＧＦ基因表达的影响。实验分６组：常氧、低氧无血清培养液组（Ｎ、Ｈ组），常氧、低氧内皮细胞条件培养液组（ＮＥＣＣＭ、ＨＥＣＣＭ组），ＮＥＣＣＭ＋ＤＤＰＨ、ＨＥＣＣＭ＋ＤＤＰＨ组。用自动图像分析仪检测各组细胞ＰＤＧＦ－Ａ和－Ｂ链杂交产物的平均光密度（ＯＤ）值。结果：ＨＥＣＣＭ组ＰＤＧＦ－Ａ和－Ｂ链ｍＲＮＡ表达量分别是ＮＥＣＣＭ组的１．４０、１．４９倍（Ｐ＜０．０１），分别是Ｎ组的１．８倍、１．７倍（Ｐ＜０．０１），ＨＥＣＣＭ＋ＤＤＰＨ组ＰＤＧＦ－Ａ、－Ｂ链ｍＲＮＡ表达量分别为ＨＥＣＣＭ的０．７３倍、０．６５倍（Ｐ＜０．０１），与ＮＥＣＣＭ组相比，均无显著差异。提示ＤＤＰＨ在ＰＤＧＦ基因转录水平抑制ＨＥＣＣＭ诱导的ＰＡＳＭＣ的增殖。

The technology of nucleic acid in situ hybridization was used to study the effect of DDPH on PDGF mRNA gene expression of pulmonary artery SMC induced by the hypoxic bovine endothelium cell condition medium(HECCM).Experimental groups included normoxic, hypoxic serum free medium group(N,H group).normoxic,hypoxic endothelium cell conditional medium groups(NECCM,HECCM groups) and NECCM+DDPH,HECCM+DDPH group.Automatic image analysis showed that HECCM substantially enhanced the expression of PDGF+A and -B chain mRNA(1.8 fold and 1.7 fold compared with N group;1.40 fold and 1.49 fold compared with NECCM group,respectively,P<0.01).HECCM+DDPH lowered the expression of PDGF-A and-B chain mRNA(0.73 fold and 0.65 fold compared with HECCM,with significant difference; but no difference was found when compared with NECCM).The results suggested that PDGF may be an autocrine growth factor of PASMC induced by HECCM and play an important role in the vascular structural remodeling of hypoxic pulmonary hypertension.DDPH could inhibit multiplication of PASMC induced by HECCM at the level of PDGF gene transcription.