海洛因对大鼠谷氨酸脱氢酶基因表达的影响

EFFECTS OF HEROIN ON GLUTAMATE DEHYDROGENASE GENE EXPRESSION IN RATS

目的:研究海洛因对大鼠谷氨酸脱氢酶(GDH)基因表达的影响。方法:40只成年♂Wistar大鼠随机分为4组:对照组腹腔注射(ip)生理盐水9d;给药3d组ip海洛因3d;给药9d组ip海洛因9d;停药组ip海洛因9d后停药8d。采用生化自动分析系统检测血浆及组织中GDH活性,逆转录聚合酶链反应法(RT-PCR)检测组织中GDHmRNA的含量。结果:血浆中GDH活性在海洛因给药期间逐渐增高,停药8d后仍无明显降低;大脑组织GDH活性在海洛因给药3d、海洛因给药9d及停药8d后均显著低于对照组(P<0·01);肝脏GDH活性在海洛因给药过程中降低(P<0·01),停药8d后恢复到对照组水平;小肠GDH活性在海洛因给药9d后显著高于对照组(P<0·05),停药8d后小肠GDH活性继续升高并明显高于对照组(P<0·01)和海洛因给药9d组(P<0·05)。大脑、肝脏和小肠GDHmRNA含量分别与其GDH活性变化趋势一致。结论:海洛因对大鼠不同组织GDH基因表达影响不同。

Objective： To determine heroin effects on gene expression of glutamate dehydrogenase （GDH） in plasma and tissues of rats . Methods： Forty adult male Wistar rats were evenly divided into 4 groups： control group was peritoneal injected （ip） normal saline for 9 days; two study groups were ip heroin for 3 days and 9 days respectively; withdrawal group was ip heroin for 9 days followed by withdrawal for 8 days. GDH activities in plasma and tissues of the four groups were detected by applying automatic biochemistry analysis system and GDH mRNA in tissues was detected by RT -PCR. Results： Plasma GDH activities increased gradually during heroin administration and remained higher than control group even after withdrawal for 8 days （P 〈0. 01 ）. GDH activities in cerebrum of 3 day and 9 day heroin administration group and withdrawal group dramatically decreased compared with control group （P 〈 0.01 ）. Liver GDH activity reduced during heroin treatment（P 〈0. 01 ） and returned to the same level as control after withdrawal for 8 days. Small intestine GDH activity increased after 9 day heroin injection（P 〈 0. 05） and kept rising after withdrawal for 8 days. GDH mRNA contents in cerebrum, liver and small intestine changed in the same way with GDH activities in corresponding tissues. Conclusion： Heroin has different effects on GDH gene expression in different tissues.