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猪Motilin和Ghrelin基因克隆及其真核载体的构建与体外表达

Cloning of Pig Motilin and Ghrelin,Construction of Recombinant pDsRed-Express-Motilin and pEGFP-Ghrelin Vector and Expression in Vitro
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摘要 目的:克隆Motilin和ghrelin基因,构建表达Motilin的pDsRed-Express-motilin载体和表达ghrelin的pEGFP-ghrelin载体,并检测它们能否在真核细胞中表达及表达量的变化。方法:用RT-PCR方法分别从猪小肠和胃的cDNA上扩Motilin和ghrelin基因片段,并分别克隆到pDsRed-Express-N1载体和pEGFP-N1载体上,用脂质体法转染RIBS2细胞系(来自于肾),并用半定量RT-PCR和荧光成像法检测目的基因的表达。结果:构建了表达Motilin的pDsRed-Express-motilin和表达ghrelin基因的pEGFP-ghrelin载体,并能在真核细胞中超表达目的基因,为进一步相关研究提供了实验基础。 Aim： clone big motilin and ghrelin gene, construct recombinant pDsRed-expree-motilin and pEGFP-ghrelin vector and their expressions in vitro. Method： motilin and ghrelin genes were amplified by PCR and were cloned to pDsRed-Express-N1 and pEGFP-N1, respectively. Then the resultant pDsRed -Express-Motilin and pEGFP-ghrelin were co-transferred into RIBS2 ceils with lipd2000. Result： construction combinations of pDsRed-Express-motilin and EGFP-ghrelin vectors are successful and their expressions are highly efficient in RIBS2 cells.
作者 应牡英
机构地区 华中农业大学生命科学院国家微生物重点实验室
出处 《江西科技师范学院学报》 2006年第6期103-106,共4页 Journal of Nanchang Vocational & Technical Techers' College
关键词 胃动素Motilin 生长激素释放肽Ghrelin 转染 RIBS2细胞系 Motilin Ghrelin infection RIBS2 cells
分类号 Q291 [生物学—细胞生物学]
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参考文献14

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江西科技师范学院学报
2006年 第6期

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