马尾松幼胚培养愈伤组织诱导的初步研究

Preliminary Report on Study of Callus Inducing from Immature Embryo in Pinus Massonian

取优良的马尾松幼胚作为外植体,在添加不同BA、NAA浓度配比的1/2MS培养基上于室温25±2℃进行组织培养.研究表明,以1/2MS为基本培养基时,进行愈伤组织诱导的最佳激素配比为1/2MS+BA1+NAA0.1+0.6%琼脂+30/L蔗糖.愈伤组织大多为白色或白绿色的颗粒状,质地疏松半透明而有光泽.NAA是诱导愈伤组织的必需因子,但量过多又不利于外植体不定芽的产生.图1,表2,参24.

The immature embryos of Pinus massonian were cultured as explants on 1/2MS medium containing various combinations of BA and NAA at 25 ± 2 ℃. The results showed that the best medium for inducting callus is 1/2MS ＋ BA1 mg/L ＋ NAA0. 1mg/L ＋ agar 0.6% ＋ sucrose 30 g/L, and NAA is necessary factor for inducting callus of the immature embryo, lfig. ,2tabs. ,24refs.