摘要
目的:了解青蒿琥酯(artesunate,ART)对角质形成细胞生长的抑制作用及机制。方法:用不同浓度ART处理人角质形成细胞株HaCat,MTT法检测ART对HaCat增殖情况的影响,流式细胞仪分析ART处理后的HaCat的趋化因子CXCR2表达的改变。对正常人表皮角质形成细胞进行原代培养,观察ART处理后,培养液中TGF-β1含量的变化。结果:0·01～0·5mg/mlART可抑制HaCat细胞的增殖,干预24小时后即可见到明显的抑制作用,但CXCR2的表达稍高于正常对照组。同时,正常原代的角质形成细胞的培养上清中TGF-β1分泌增加。结论:0·01～0·5mg/ml青蒿琥酯可促进角质形成细胞TGF-β1分泌,抑制角质形成细胞的增殖。
Objective: To investigate the effect of artesunate on the CXCR2 expression and TGFβ1 secretion content in cultured keratinocytes. Methods: The keratinocyte cell line HaCat was cultured with artesunate at variousconcentration, the proliferation of cells was detected by MTT method. The expression of CXCR2 was assayed by flow cytometry. The secretion content of TGFβ1 in the cultured keratinocyte was examined by ELISA. Results: Artesunate could obviously inhibit the proliferation of keratinocyte at concentration of 0.01～0.5mg/ml.The expression of CXCR2 in HaCat cells was slightly up-regulated, the secretion of TGFβ1 was enhanced in keratinocyte cultured by artesunate. Conclusion: Artesunate could suppress the proliferation of keratinocyte by regulating the expression of CXCR2 and enhancing the secretion of TGFβ1 in vitro.
出处
《中国中医药科技》
CAS
2007年第2期91-92,共2页
Chinese Journal of Traditional Medical Science and Technology
基金
安徽省教育厅基金资助课题No.kj2005zd
