毛白杨纤维素合酶基因(PtoCesA1)的克隆及其在烟草中的遗传转化

Isolation and Transformation in Nicotiana tabacum of a Cellulose Synthase Gene (PtoCesA1) from Populus tomentosa

克隆毛白杨纤维素合酶基因（PtoCesAl），全长为3215bp，与欧洲颤杨的PtrCesAl基因的同源性为97％，具有开放的阅读框，编码区在52～2988碱基之间，为2937bp。构建PtoCesAl基因的全长正义植物表达载体为pBIPAl，经酶切和PCR鉴定确认载体构建正确。通过农杆菌介导的方法将pBIPAl表达载体转入烟草中，转基因植株的纤维细胞壁厚度和木质部厚度都有不同程度的下降，形态表征为植株明显变矮，叶片变小。

A cellulose synthase gene（ PtoCesAl ）, isolated from Populus tomentosa, was 3 215 bp long with an opening reading frame of 2 937 bp extending from nucleotides 52 - 2 988. Comprison of the nucleotide sequence of PtoCesAl with PtrcesAl from Populus tomentosa showed 97% identity. The full length PtoCesAl cDNA was subeloned into pBI121 and identified by digestion, then transformed into Nicotiana tabacum by leaf disc. The transgenic plants with PtoCesAl had small leaves, and ＂dwarf＂ phenotype, consistent with a loss of xylem and fiber cell wall thickness