绿玉树春季乳汁的碳氢化合物及甾醇的GC-MS分析

Analysis of Hydrocarbon and Sterol in Spring Latex of Euphorbia tirucalli by Gas Chromatography/Mass Spectrometry

取西双版纳的成年绿玉树和重庆引种的2年生海南绿玉树乳汁，自然干燥后用CS2溶解，经气相色谱-质谱联用机（GC-MS）分析，西双版纳绿玉树乳汁共检出52个峰，鉴定出33个峰，23种成分。除绿玉树的“指纹”成分绿玉树甾醇以外，还含有9～36个碳原子的多种烷烃，如2，6-二甲基壬烷、3，7-二甲基癸烷、十二烷、二十烷、正二十烷、二十一烷、二十三烷、二十五烷、二十七烷、三十烷、三十六烷、石竹烯以及1，2-苯二羧酸、酯类和单质硫（S8）等，其中烷烃和烯烃的总相对含量为14．57％；海南幼年绿玉树乳汁有42个峰，鉴定出33个峰，21种成分。除绿玉树甾醇外，主要也含9～26个碳原子的烷烃，如壬烷、十六烷、十七烷、十八烷、二十烷、正二十烷、二十一烷、二十二烷、β-芘澄茄烯、异石竹烯、大根香叶烯-D以及1，2-苯二羧酸、酯类和单质硫（S8）等，其成分与西双版纳绿玉树十分接近，但绿玉树的“指纹”成分绿玉树甾醇的含量（17．40％）要稍高于西双版纳成年绿玉树（10．91％），而烷烃和烯烃的相对含量（5．01％）要低于西双版纳成年绿玉树（14．57％）。

The latex of Euphorbia tirucalli from Xishuangbanna and Hainan Island were analysed by agilent gas chromatography/mass spectrometry. 52 peaks （42 peaks, E. tirucalli from Hainan） were seperated and 33 peaks were identified, the latex contains as hydrocarbons normal and isomeric alkanes with carbon numbers from 9 - 36, eg, 2,6-dimethyl-nonane, 3, 7-dimethyl-decane, hexatriacontane, pentadecane, dodecane, heptadecane, eicosane, tricosane, heneicosane, triacontane, docosane, N-eicosane, caryophyllene, 1,2-benzenedicarboxylic acid, ester and sulfur etc, except main constitutents ＂fingerprint＂ Lanosta-8,24-dien-3-ol. The alkanes represent 14.57% of the total content. The components of latex not exist in significant differences between two E. tirucaUi from Xishuangbannan and Hainan Island. The main components of younger tree from Hainan also contain as hydrocarbons normal and isomeric alkanes with carbon numbers from 9 - 36, eg, eicosane, 2,4-dimethyl-, octadecane, cyclohexasiloxane, dodecamethyl, nonadecane, pentaco-sane, heneicosane, eicosane, hexadecane, heptadecane, docosane, N-eicosane, beta-cubebene, caryophyllene, germacrene-D, 1,2-benzenedicarboxylic acid, ester and sulfur compound etc, but the content of Lanosta-8,24-dien-3β-ol （17.40%） reprsents higher than the content of adult tree （10.91%）from Xishuangbanna, lower than adult tree on alkanes, the alkanes of younger tree represent 4.86 %, adult tree represent 14.57 % of the total content.