PTEN、VEGF蛋白表达与犬乳腺肿瘤病理学特征的关系

Relation between expressions of PTEN and VEGF and clinicopathological features in canine mammary gland tumors

为探讨犬的乳腺肿瘤组织中PTEN和VEGF蛋白表达与临床病理学特征关系的相关性,采用免疫组织化学SP染色法检测了32例乳腺肿瘤组织及6例正常乳腺组织中上述2种蛋白的表达。结果发现:1)PTEN蛋白在正常乳腺组织和良性乳腺肿瘤高表达率为100%,在恶性乳腺肿瘤组织中的高表达率为67%(8/12),两者比较差异极显著(P<0.01);乳腺肿瘤组织中PTEN蛋白表达与犬的年龄、肿瘤大小无关(P>0.05),而与淋巴结转移、组织学分级有关(P<0.01)。2)VEGF蛋白在正常乳腺组织中高表达率为33.3%(2/6),在乳腺肿瘤组织中高表达率为78%(25/32),两者比较差异极显著(P<0.01);乳腺肿瘤组织中VEGF蛋白表达与患病犬的年龄、肿瘤大小无关(P>0.05),而与淋巴结转移有关(P<0.05)。联合检测PTEN、VEGF表达可作为乳腺癌生物学行为和预后判断的重要指标。

To investigate the relationship between expression of PTEN and VEGF and clinicopathological features in canine mammary gland tumor, the expression levels of PTEN and VEGF protein were assessed in 32 cases of canine mammary gland tumors tissues and 6 cases of normal mammary gland tissues using SP immunohistochemical method. The over-expression rate of PTEN protein was 100% in normal and well- differentiated mammary gland tissues and 67% in breast cancer cases respectively with a significant difference between the two groups （P〈 0.01）. Expression of PTEN was not related to age and tumor size, but closely cbrrelated with lymph node metastasis （P 〈 0.01）. The over-expression rate of VEGF protein was 33.3% in normal mammary gland tissues, and 78% in canine mammary gland tumors with a significant difference between the two groups （P〈0.01）. Expression of VEGF was not related to age or tumor size, but closely correlated with lymph node metastasis and clinical stage （P 〈 0.05）. Therefore, the combined detection of PTEN and VEGF could serve as an important index to estimate the biological behavior and prognosis of canine mammary gland tumors.