摘要
目的探讨胎盘组织中血红素氧合酶(HO)和内源性一氧化碳(CO)在妊娠期高血压疾病(Hypertensive disorder complicating pregnancy,HDCP)发病中的作用及相互关系。方法采用免疫组织化学和RT-PCR法检测15例正常晚孕和40例妊娠期高血压疾病患者胎盘组织中HO-1、HO-2蛋白表达以及mRNA转录水平,分光光度法检测胎盘组织匀浆上清液碳氧血红蛋白(COHb)含量。结果HO-1蛋白主要定位于胎盘滋养细胞、血管内皮细胞和间质细胞,HO-2蛋白主要表达于胎盘滋养细胞及血管内皮细胞;正常妊娠组胎盘HO-1、HO-2mRNA表达均较强,平均灰度值分别为1.13±0.19、1.05±0.68,HO-1mRNA、蛋白表达及胎盘组织COHb含量在妊娠期高血压疾病中最强,随妊娠期高血压疾病病情的加剧而逐渐减少,与正常组比较有显著差异(P<0.05,P<0.01);而HO-2在正常妊娠和妊娠期高血压疾病组间无明显区别(P>0.05);胎盘组织中HO-1表达与内源性CO浓度呈显著正相关(r=0.48,P<0.05)。结论妊娠期高血压疾病患者胎盘HO-1蛋白表达和转录降低、内源性CO生成减少,HO/CO系统可能参与了妊娠期高血压疾病发病过程。
Objective： To investigate the expression of hemeoxygenase - 1 and hemeoxygenase 2 in human placenta tissue, and the relationship between hemeoxygenase and carbon monoxide and pregnancy induced hypertensive （PIH） . Methods： Immunohistochemistry and reverse transcription polymerase chain reaction （RT- PCR） were resorted to demonstrate the expression HO- 1 and HO- 2 in normal and PIH placenta tissue, COHb in placenta tissue was determined by spectrophotometry. Results： The results of immunohistochemistry demonstrated that HO - 1 was predominantly localized trophoblast and villous stroma cell, HO -2 predominantly localized in trophoblast capillaries with weak staining of villous stroma; the expression of HO - 1 （ 1. 13 ± 0. 19）, and HO - 2 （ 1.05 ± 0. 68 ） was higher in control group, HO - 1 mRNA, protein and COHb were the highest in gestational hypertension ; but decreased to follow the progress of PIH, there was significant difference among PIH groups （ P 〈 0. 05 ）, but HO - 2 was no significant difference among groups （ P 〉 0. 05 ） ; The expression of HO - 1 correlated with the concentrations of CO in placental tissues （ r = 0. 48, P 〈 0. 05 ）. Conclusion： The expression level of HO - 1 protein and transcript decrease accompanying with endogenous CO reduction in placenta are correlated with the progress of HIP HO and CO system contributed to the pathogenesis of HIP.
出处
《中国妇幼保健》
CAS
北大核心
2007年第12期1649-1651,共3页
Maternal and Child Health Care of China
