腹腔注射IL-2对小鼠单核吞噬细胞吞噬功能的影响

Influence of intraperitoneal injection of IL-2 on mononuclear phagocyte phagocytosis in mice

目的观察不同浓度IL-2对小鼠单核吞噬细胞吞噬功能的影响。方法取20只健康昆明小鼠随机分为五组:正常对照组和4个不同浓度的实验组。将浓度分别为20,40,80和160 U/ml的IL-2(实验组)经腹腔注入小鼠体内,观察小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬能力,计算出不同浓度IL-2作用后吞噬细胞的吞噬百分率和吞噬指数,并与正常对照组作比较。结果正常对照组小鼠巨噬细胞吞噬百分率为(16.98±1.22)%,20 U/ml组为(39.66±0.98)%,40 U/ml组为(57.61±1.04)%,80 U/ml组为(60.22±1.32)%,160 U/ml组为(61.11±0.99)%;吞噬指数依次为0.21±0.048,0.52±0.032,0.79±0.041,0.82±0.057和0.83±0.028。实验组与正常对照组之间具有显著性差异(P<0.05)。结论腹腔注射IL-2可以显著增强小鼠单核吞噬细胞的吞噬功能。

Objective To study the influence of different concentrations of IL-2 intraperitoneally injected into mice on mononuclear phagocyte phagocytosis. Methods Twenty healthy mice were divided into five groups randomly： normal control group and four experiment groups. The mice were intraperitoneally injected with 20,40,80 and 160 U/ml IL-2 in four experiment groups, respectively. The mononuclear phagocyte phagocytosis was observed, and phagocytic percentage and phagocytic index were calculated. Results The phagocytic percentages were（16.98 ± 1.22） % in normal control group, and（39.66 ±0.98） %, （57.61 ±1.04） %, （60.22 ±1.32） %, （61.11 ±0.99） % in experiment groups, respectively. The phagocytic index was 0.21 ± 0. 048 in normal control group, and 0.52 ± 0. 032, 0.79 ±0.041,0.82 ± 0. 057 and 0.83 ± 0. 028 in experimental groups, respectively. There was significant difference between experiment groups and normal control group （P 〈 0.05）. Conclusion Injecting intraperitoneally IL-2 into mice can significantly strengthen mononuclear phagocyte phagocytosis.