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MTT法在检测细胞增殖方面的探讨 被引量:91

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摘要 目的了解MTT(四甲基偶氮唑盐)法在检测细胞增殖性方面的作用。方法采用酶标仪在570 nm波长附近(500-600 nm)处测定光吸收值,以反映活细胞的数目。结果吸光度值在0.75-1.25时,这样才能保证数据有线性关系,MTT比色法才可灵敏、准确地反映出细胞数量上的变化。结论MTT比色法是一种检测细胞存活和增殖性方面有效的方法,具有操作简便、经济、快速、易自动化的优点。
出处 《山西医科大学学报》 CAS 2007年第3期262-263,共2页 Journal of Shanxi Medical University
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  • 8方蓉,李芳秋,武建国.MTT比色法的条件探讨[J].临床检验杂志,2003,21(1):34-35. 被引量:66

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