CAP系统检测过敏原特异性IgE抗体的方法学评价

Evaluation of CAP test system for allergen-specific IgE assay

目的评价CAP过敏原检测系统检测过敏原特异性IgE抗体的准确性和预测值。方法研究对象为2003～2004年就诊的病例,结合病史和体内试验结果做出过敏原特异性的临床诊断。对其中明确诊断为尘螨、蒿属花粉、葎草花粉、猫毛屑或链格孢霉5种过敏原过敏者用CAP过敏原检测系统进行相应的过敏原特异性IgE检测,并随机选取部分临床明确诊断对上述5种过敏原不过敏者同时进行相应过敏原特异性IgE检测,分析CAP系统检测的准确性并计算其阳性预测值和阴性预测值。结果共进行了957项次过敏原特异性IgE检测,对尘螨、蒿属花粉、葎草花粉、猫毛屑、链格孢霉特异性IgE检测的灵敏度分别为99.0%、97.9%、91.7%、93.5%、85.5%,特异性分别为96.2%、95.8%、93.0%、97.0%、91.7%。特异性IgE检测结果与临床诊断之间的符合率较高,阳性预测值均大于94%,阴性预测值均大于80%。结论用CAP过敏原检测系统检测过敏原特异性IgE抗体的结果准确性好,预测值高,表明该方法具有较高的实用诊断价值。

Objective To evaluate the validity and predictive value of CAP specific IgE test system. Methods Five allergen-specific IgEs, i.e. , specific IgE to mites, mugwort pollens, hop pollens,cat epithelium and dander, and Alternaria alternate,were tested in selected randomly allergic and non-allergic patients by using CAP system. Results In 957 tests the sensitivity of specific IgE to the 5 allergens was 99.0% ,97.9% ,91.7% ,93.5% and 85.5% ,and the specificity was 96.2% ,95.8% ,93.0% ,97.0% and 91.7%. The positive and negative predictive values were greater than 94% and 80% respectively. Conclusion CAP system is of great potential value for diagnosis of allergenas.