转phaC叶绿体型烟草的光学属性

Optical Properties of phaC-Transgenic Tobacco

为探讨光谱无损测试技术在转phaC叶绿体型烟草材料筛选中应用的可行性,对烟草品种Wisconsin38(Nic-otiana tobacum cv·Wisconsin38,对照)和其转phaC基因烟草无损活体测定了相对叶绿素含量(SPAD)、叶绿素荧光参数和反射率,并对测定结果进行了分析.结果表明:(1)PSⅡ有效光化学量子产量[(Fms-Fs)/Fms]和SPAD均表现为对照烟草大于转基因烟草,其中,(Fms-Fs)/Fms差异显著.(2)对照的反射率光谱大于转基因烟草.两者在350～690nm、750～1350nm和2113～2320nm反射率变异显著.(3)一些波长反射率与PSⅡ最大光化学量子产量(Fv/Fm)、有效光化学量子产量(Fms-Fs)/Fms和SPAD值达到显著相关.(4)光谱指数中,D730/D706与SPAD值相关系数达到了0·833.Area1190、P_Nd1280、R685/R630、(R683)2/(R675×R691.)与(Fms-Fs)/Fms达到显著相关.上述结果表明高光谱等无损测试技术在基因工程中应用是一个值得重视的方向.

In order to investigate the feasibility of applying hyper-spectrum non-destruction technique in the screening of phaC-transgenic tobacco germplasms, non-destruction and in vivo measurements of the relative chlorophyll content （SPAD） and chlorophyll fluorescence and reflectance of Nicotinana tobacum cv.Wisconsin 38 and its phaC-transgenic counterpart were determined non-destructively. The yield of quantum efficiency [（Fms-Fs）/Fms] and SPAD were significantly greater in Wisconsin 38 than in phaC-transgenic tobacco. Plant reflectance in Wisconsin 38 was greater than that in phaC-transgenic tobacco, the difference being significant in the visible and infrared regions of 350-690 nm, 750-1350 nm and 213-2 320 nm. In some wavelength regions, significant correlation coefficients were recorded in reflectance and maximal photochemical efficiency （Fv/Fm）, [-（Fms-Fs）/Fms] and SPAD. The correlation coefficient between D730/D706 and SPAD was 0. 833. （R638）2/（R675×R691） was the best index for its significant correlation with chlorophyll fluorescence and SPAD. These results suggest that the application of non-destructive techniques like hyper-spectrum in genetic engineering merits further study.