诊断为慢性非细菌性前列腺炎患者的前列腺液和前列腺组织中细菌基因的检测

Detection of bacterial gene in express prostate secretions and prostate biopsies of patients with chronic bacterial prostatitis

【目的】探讨细菌感染在诊断为慢性非细菌性前列腺炎患者中的作用。【方法】应用PCR方法检测38例慢性非细菌性前列腺炎患者的前列腺液和前列腺组织中细菌16SrRNA基因,用尿道拭子和直肠拭子以及穿刺枪头拭子作对照。然后用RT-PCR方法检测细菌16SrRNA基因阳性的对应前列腺组织的大肠杆菌tufAmRNA基因。【结果】细菌16SRNA基因的检出率在前列腺液中和前列腺组织中分别为78.9%和81.5%(P>0.05)。细菌基因信号在前列腺液标本中和尿道拭子中各有30例(78.9%)和4例(10.5%)呈阳性(P<0.01);在前列腺组织中和直肠拭子中各有31例(81.5%)和6例(15.8%)呈阳性(P<0.01),无1例穿刺枪头拭子阳性。在31例细菌16SrRNA基因阳性的患者前列腺组织中,有6例(19.4%)检出大肠杆菌tufAmRNA基因。【结论】诊断为慢性非细菌性前列腺炎患者的前列腺液和前列腺组织中不仅有细菌的存在,而且部分被证实是活力细菌。细菌在此类患者的病因中可能起一定的作用。

[Objective] To investigate the role of bacteria in chronic abacterial prostatitis. [Methods] Polymerase chain reaction （PCR） was used to detect the bacterial 16SrRNA gene signal in EPSs and prostate biopsies of 38 men with chronic abacterial prostatitis. Also, urethral swabs, biopsy gun needle swabs and rectal swabs were assayed by the same technique. After that, Reverse transcriptase （RT） -PCR was used to detected E. Coli tufAmRNA gene of these positive prostate biopsies. [Results] Bacterial 16SrRNA gene detection rates in EPS samples and prostatic biopsies were 78.9% andS1.5%, respectively （P 〉 0.05）. Bacterial 16SrRNA gene was detected in 30 （78.9%） EPS samples and 4 （10.5%） urethral swabs （P〈O.O1）; 31 （81.5%） prostatic biopsies and 6 （15.8%） rectal swabs （P〈O.O1）. The result was negative in all needle swabs. E. Coli tufAmRNA gene were detected in 6 （19.4%） samples of 31 samples with positive 16SrRNA gene. [Conclusion] Bacterial not only existed in EPS and prostate tissue of patients with chronic abacterial prostatitis, but also partly viable. Bacterial infection may play a role in the etiology of chronic abacterial prostatitis.