摘要
目的研究消风散对2、4-二硝基氯苯(DNCB)诱导小鼠Ⅳ型超敏反应的影响。方法建立小鼠Ⅳ型变应性接触性皮炎(ACD)模型,观察消风散对小鼠耳肿胀、外周血白细胞数的影响,采用ELISA法检测小鼠血清中的白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)及可溶性白细胞介素-2受体(sIL-2R)的含量。结果消风散可改善DNCB引起的小鼠耳部肿胀,明显降低末梢血中白细胞总数,抑制IFN-γ及sIL-2R水平,提高IL-4含量。结论消风散能抑制Ⅳ型超敏反应,作用机制与抑制白细胞总数、调节细胞因子及受体有关。
Objective To study the effects of Xiaofeng Powder on IL-4, IFN- γ and sIL-2R in delayed hypersensitivity and to explore the mechanism of treatment. Methods The mice model of allergic contact dermatitis was formed by DNCB. The effect of Xiaofeng Powder on ear swelling and leukocyte counts of the model mice were observed. The serum levels of interleukin-4 (IL-4), interferongamma (IFN-γ) and soluble interleukin-2 receptor (sIL-2R) of the mice were detected by enzyme-linked immunosorbent assay (ELISA). Results Xiaofeng Powder could inhibit ear sweUings and obviously reduced the total amount of leucocytes. Xiaofeng Powder could heighten IL-4 level and reduce the level of IFN-y, sIL-2R. Conclusion Xiaofeng Powder has the function of inhibiting delayed hypersensitivity. The mechanism of treatment may be related to inhibiting the total amount of leucocytes, regulating cell factor and receptor.
出处
《中国中医药信息杂志》
CAS
CSCD
2007年第4期37-38,共2页
Chinese Journal of Information on Traditional Chinese Medicine