活血胶囊中丹参素和原儿茶醛的含量测定

Determination of Danshensu and Protocatechuic Aldehyde in Huoxue Capsule

目的制定活血胶囊中丹参素和原儿茶醛的含量测定方法。方法采用HPLC法,以丹参素和原儿茶醛为对照品,检测波长为280nm,甲醇-0.5%醋酸水溶液(22∶78)为流动相。结果丹参素在0.421～3.364μg范围、原儿茶醛在0.212～1.699μg范围有良好的线性关系,样品平均加样回收率丹参素和原儿茶醛分别为100.05%和99.39%,RSD分别为3.07%和1.62%。结论本法结果准确,精密度及重现性较好,可作为该制剂的质量控制方法。

Objective To establish a method for determining the content of Danshensu and Protocatechuic Aldehyde in Huoxue capsule. Methods The HPLC method was adopted for the determination. Danshensu and Protocatechuic Aldehyde were used as the standard. Detection wave length was set at 280 nm, the mobile phase consisted of methanol-0.5 % HAc （30 ： 70）. Results The linear range of Danshensu was 0.421-3.364 μg and Protocatechuic Aldehyde was 0.212-1.699 μg. The average recovery rate of Danshensu was 100.05% with RSD of 3.07% and Protocatechuic Aldehyde was 99.39% with RSD of 1.62%. Conclusion This method is accurate, specific and reliable for the quality control of Huoxue capsule.