沙棘SSR分子标记的开发

Exploitation of SSR Marker of Hippophae rhamnoides

利用已报道的葡萄SSR引物筛选适用于沙棘的SSR引物。结果表明，在供筛选的107对引物中，只有1对引物在沙棘中无扩增位点，有效扩增比率为99．07％；共有46对引物的在2个沙棘品种间扩增出多态性位点，有效扩增比率为42．99％，扩增产物共表现出100个等位基因，其中差异等位基因数为70个，每对引物的等位基因数为1～4个，平均为2．174个。根据这46对引物的扩增结果，中国沙棘和辽阜1号间的遗传相似系数为0．413，遗传距离为0．587。

SSR markers adapting to Hippophae rhamnoides were screened using reported Grape＇s SSR primers. The results showed that among 107 primers selected only 1 primer failed amplification, with success rate 99.07%. 46 primers showed polymorphism, with success rate 42,99%, Total 100 alleles were gotten according to their amplification, including 70 aberrant alleles, Allele number of each primer ranged from 1 to 4 with a mean of 2.174. The genetic similarity coefficient between two varieties was 0.413, and the genetic distance was 0.587,