过氧化物酶体增殖物激活受体基因多态性与Ⅱ型糖尿病患者的影响

Relationship between Peroxisome proliferator-activated receptor gamma2 Pro12Ala Gene Polymorphism and Diabetes Mellitus Patient

目的:研究过氧化质体增殖物激活受体(Peroxisome proliferator-activated receptor,PPAR)γ2基因Pro12Ala多态性是否与Ⅱ型糖尿病(IDDM)的易感性相关。方法:通过PCR-RFLP方法,对275例糖尿病患者和286例正常人进行了PPAR基因Pro12Ala多态性分析,比较各组间等位基因型及等位基因频率的分布。结果:研究对象中共见有2种基因型,Pro/Ala杂合子等位基因型,Pro/Pro纯合子等位基因型。IDDM患者基因型Pro/Ala、Pro/Pro频率分别为10%、90%,x2=3.39,P>0 05,而对照组Pro/Ala、Pro/Pro频率分别为5%、95%;IDDM患者等位基因Pro和Ala频率分别为95%、5%,x2=3.27,P>0 05,而对照组Pro和Ala频率分别为98%、2%。结论:糖尿病患者与对照组之间Pro12Ala多态性频率存在差异,但不显著。PPARγ2基因Pro12Ala多态性与Ⅱ型糖尿病的易感性可能无关。

Objective： To investigate whether Peroxisome proliferator - activated receptor （PPAR） γ2 gene Pro12Ala polymorphism is associated with the susceptibility to diabetes mellitus （DM）. Methods： In this experiment, we analyze the PPARγ2 gene Pro12Ala polymorphism in 561 Chinese subjects including 275 patients with IDDM and 286 control subjects by the PCR - PFLP method. The genotype frequencies for Pro/Ala,Pro/Pro were 10% and 90% , respectively, in the DM group ,and 5% ,95% in the control subjects. Results： The Pro/Ala frequency is not significantly higher in the patients than in the control group （P 〉 0.05）, and so is the Ala allele, suggesting that the Pro/Ala genotype maybe not associated with the susceptibility to IDDM in our study group. Conclusions ： There maybe no correlation between PPARγ2 gene Pro12Ala polymorphism and susceptibility to IDDM.