白细胞介素-4、-10对大鼠肾缺血再灌注损伤细胞凋亡的抑制作用

Inhibitive Effect of Interleukin-4,-10 on Cell Apoptosis in Rats with Renal Ischemic-Reperfusion Injury

目的探讨白细胞介素-4(IL-4)和(或)IL-10对大鼠肾缺血再灌注损伤(IRI)是否有抑制细胞凋亡的作用及其机制。方法雌性SD大鼠30只随机分为5组(n=6):假手术组;手术+9g/L盐水对照组(NaCl组);手术+IL-4组(IL-4组);手术+IL-10组(IL-10组);手术+IL-4+IL-10组(IL-4+IL-10组)。用无损伤动脉夹钳夹大鼠双侧肾蒂45min制成肾IRI,钳夹松开时,各组迅速注射相应的IL或9g/L盐水,12h后取血及摘除左肾做病理切片。观察肾脏上皮细胞凋亡数(TUNEL法)。结果IL-4组肾脏上皮细胞凋亡水平无降低。IL-10组肾脏上皮细胞凋亡减轻。而联合运用IL-4+IL-10明显抑制大鼠肾脏上皮细胞凋亡。结论联合运用IL-4+IL-10明显抑制大鼠肾脏上皮细胞凋亡,对肾脏上皮细胞结构及功能有保护作用。

Objective To study the inhibitive effect of interleukin -4 （IL- 4 ） and （or） IL - 10 on cell apoptosis of renal ischemic - reperfusion injury（ IRI ） in rats and its mechanism. Methods Thirty female Sprague - Dawley rats were divided into five groups （ n = 6 ） ： sham - operated group, operation ＋ 9 g/L NaCI control （ NaCI group） , operation ＋ IL - 4 （ IL - 4 group） , operation ＋ IL - 10 （ IL - 10 group ）, operation ＋ IL -4 ＋ IL - 10 （ IL -4 ＋ IL - 10 group）. The animal model of IRI was set up by clamping bilateral renal pedicles for 45 min. When the clamp was loosed, relevant agents was injected in every group. Apoptosis of renal epithelial cells （TUNEL method） was obserued. Results IL - 4 could not reduce level of apoptosis of renal epithelial cells. To some extent, IL - 10 could reduce level of renal epithelial cells. The combination of IL - 4 and IL - 10 was more effective in inhibiting apoptosis of renal epithelial cells. Conclusion The combination of IL - 4 and IL - 10 is effective in inhibiting apoptosis of renal epithelial cells, indicating that it has a relationship in preventing renal histological and functional damage.