全身炎症反应综合征患儿血内毒素、脂多糖结合蛋白/脂多糖受体水平变化的意义

Changes of Hematoxin,Lipopolysaccharide,Lipopolysaccharide Binding Protein/Membrane CD_(14) in Children with Systemic Inflammatory Response Syndrome

目的研究血内毒素(LPS)、脂多糖结合蛋白(LBP)/脂多糖受体(mCD14)在全身炎症反应综合征(SIRS)发生发展过程中的作用。方法随机选取SIRS患儿30例,健康对照组21例。测定二组患儿血LPS、LBP、mCD14水平,LPS采用鲎试剂动态比浊定量测定法,LBP采用酶联免疫法,mCD14采用流式细胞分析技术。结果SIRS组血浆LBP水平、外周血单核细胞mCD14平均荧光强度(MFI)、血LPS水平较对照组均显著增高(Pa<0.001),且LBP、mCD14随血LPS水平的升高而增高,二者呈明显正相关(r=0.578 Pa<0.001)。结论LPS、LBP/mCD14参与SIRS的发病过程,LPS可上调LBP/mCD14的表达,LBP/mCD14表达的上调可能是机体增敏LPS作用的分子机制。

Objective To investigate the effect of hematoxin,lipopolysaccharide（LPS） ,lipopolysaccharide binding protein（ LBP）/membrane CD14 （mCD14） in occurrence and development of systemic inflammatory response syndrome（SIRS） in children. Methods Serum LPS, LBP, mean fluorescence intensity （MFI） of mCD14 on monocytes of 30 patients with SIRS were measured,at the same time 21 healthy children had been chosen to serve as control group. Results Compared with control group ,the serum LPS,LBP, MFI of mCD14 on monocytes of patients with SIRS were significantly higher（ Pa 〈 0.001 ）. And with the increasing of serum LPS concentration, LBP/mCD14 increased, the two show marked positive correlation（r =0. 710 P 〈0. 001 ）. Conclusion LPS, LBP/mCD14 play an important role in the pathophysiological process of SIRS. LPS can increase LBP/mCD14 expression ,and the expression of LBP/mCD14 increase is a machnism for LPS function.