摘要
【目的】通过观察青藤碱对人环氧化酶(COX)、白介素-1β(IL-1β)基因表达的影响,探讨青藤碱的抗炎作用机理。【方法】分离正常人外周血单核细胞(PBMC),体外加药培养,提取细胞总核糖核酸(RNA),采用半定量逆转录聚合酶链反应(RT-PCR)技术,观察青藤碱对脂多糖(LPS)刺激和非LPS刺激状态下环氧化酶及IL-1β基因表达的影响。【结果】青藤碱对COX-1基因表达无明显影响,对COX-2基因表达仅有轻微的抑制作用,而对IL-1β基因表达的抑制作用较强。【结论】青藤碱可能是通过抑制炎症性细胞因子的产生而发挥其抗炎作用。
[Objective] The effect of sinomenine on human cyclo-oxygenase (COX) and interleukin-1β (IL-1β) was observed to explore the anti-inflammatory mechanism of sinomenine. [Methods] Peripheral blood monocytes (PBMC) were isolated and incubated with sinomenine. After incubation, total RNA from PBMC was extracted and the effect of sinomenine on COX and IL-1β expression with or without the stimulation of lipopolysaccharide (LPS) was observed by the method of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). [ Results] Sinomenine had no effect on COX-1 gene expression, had a slight inhibitory effect on COX-2 gene expression and had a strong inhibitory effect on IL-1β gene expression. [Conclusion] The anti-inflammatory mechanism may be related to the inhibition of inflammatory cytokines.
出处
《广州中医药大学学报》
CAS
2007年第2期141-143,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
关键词
青藤碱/药理学
炎症/中药疗法
基因表达调控
细胞培养
SINOMENINE/pharmacology
INFLAMMATION/TCD therapy
GENE EXPRESSION REGULATION
CELL CULTURE