多种中药单体对人结肠癌HT-29细胞株TLR4 mRNA表达和IL-8分泌的影响

Effect of Chinese Herbal Active Ingredients on TLR4 mRNA Expression and IL-8 Secretion in HT-29 Cell Line

【目的】观察不同中药单体对人结肠癌细胞株HT-29细胞Toll样受体信使核糖核酸(TLR4 mRNA)表达及白细胞介素8(IL-8)分泌的影响。【方法】选用人结肠癌细胞株HT-29细胞,采用逆转录聚合酶链反应(RT-PCR)检测HT-29细胞的TLR4 mRNA的表达;酶联免疫吸附法(ELISA)检测HT-29细胞的IL-8分泌。【结果】黄芪甲苷组和大黄素组可抑制干扰素-γ(IFN-γ)诱导的TLR4 mRNA表达,而姜黄素组、柚皮苷组和栀子苷组则对TLR4 mRNA表达无显著性影响;大黄素能显著性降低IFN-γ+脂多糖(LPS)所诱导的HT-29细胞IL-8的产生。【结论】大黄素抗炎细胞分子机制可能与其能抑制IFN-γ诱导的HT-29细胞TLR4 mRNA表达和IFN-γ+LPS诱导的IL-8分泌有关。

[Objective] To observe the effect of different active ingredients from Chinese herbal medicine on interleukin 8 （IL-8） secretion and Toll-like receptor 4 （TLR4） mRNA expression in human intestinal tumor cell （HT-29） line. [ Methods] Reverse transcription polymerase chain reaction （RT-PCR） was used to detect TLR4 mRNA expression, and enzyme-linked immunosorbent assay （ELISA） was used to examine IL-8 secretion. [Results] Astragaloside and emodin inhibited TLR4 mRNA expression stimulated by interferon γ （IFN-γ）, and emodin decreased IL-8 secretion stimulated by IFN-γ and lipopolysaccharide （LPS）. However, curcumin, naringin and jasminoidin had no effect on TLR4 mRNA expression. [ Conclusion] The anti-inflammatory cellular mechanism of emadin may be related to the inhibition of TLR4 mRNA expression stimulated by IFN-γ, and to the inhibition of IL-8 secretion stimulated by IFN-γ and LPS in HT-29 cell line.