枫香槲寄生化学成分及抗肿瘤活性研究

Studies on Chemical Constituents and Antitumor Activity of Viscum liquidambaricolum Hayata.

[目的]研究枫香槲寄生的化学成分，寻找抗肿瘤的活性成分。[方法]采用色谱技术对枫香槲寄生的化学成分进行分离、纯化，根据理化性质、波谱数据对所获得化合物进行结构鉴定，并对所获2种三萜及1种甾醇类化合物进行体外抗肿瘤活性筛选。[结果]分离并鉴定出4个化合物（化合物1～4），分别为芜花素、β-香树脂醇乙酸酯、古柯二醇、β-谷甾醇，其中β-香树脂醇乙酸酯、古柯二醇对小鼠肉瘤腹水株（S180）、小鼠艾氏腹水瘤株（EAC）、小鼠肝癌腹水株（HeAP），B谷甾醇对S180、EAC瘤株均具有一定的抑制活性，其半数抑制浓度均小于400μg／mL。[结论]芜花素、β-香树脂醇乙酸酯、古柯二醇、β-谷甾醇均为首次从枫香槲寄生中分离得到，其中芜花素为首次从桑寄生科中获得；枫香槲寄生中的三萜及甾醇类化合物具有一定的抗肿瘤活性。

[ Objective ] The chemical constituents of Viscum liquidambaricolum Hayata. were studied to screen the constituents with antitumor activity. [ Methods ] Chromatography was used for the isolation and purification of chemical constituents of Viscum liquidambaricolum Hayata.. And their structures were identified on the basis of spectroscopic evidences and physiochemical properties. Meanwhile, the in-vitro antitumor activities of two kinds of triterpenoids and one kind of sitosterol were investigated. [ Results ] Four compounds were isolated from Viscum liquidambaricolum Hayata. and their structures were identified as genkwanin （1）β-amyrin acetate （2）, erythrodiol （3） and β-sitosterol （4）. Compounds 2 and 3 had an inhibitory effect on mice sarcoma 180 （S180）, Ehrlich ascites carcinoma （EAC） and ascties hepatoma （HEAP）, and compound 4 had an inhibitory effect on mice S180 and EAC, their half-inhibitory concentrations being less than 400μg/mL. [Conclusion] Compounds 1 - 4 are isolated from Viscum liquidambaricolum Hayata. for the first time, and compound 1 is isolated from Loranthaceae for the first time. Triterpenoids and steroids from Viscum liquidambaricolum Hayata. exert certain antitumor activity.