摘要
A piezoelectric immunoassay based on nanoprobe immunoagglutination was proposed for rapid and sensitive detection of IgG. The nanoprobes were prepared by labeling gold nanoparticles with anti-IgG antibody. And a piezoelectric sensor was used to monitor the changes in solution density and viscosity caused by the immunoagglutination of IgG and anti-IgG nanoprobes. The effects of pH and ionic strength were investigated. It is found that the developed immunoagglutination assay is sensitive to IgG with the detection limit of 1.12, 0.72, 0.70 nmol/L to the detection time of 10, 20, 30 min, respectively, and it is unnecessary for immobilization of immunoactive entities.
A piezoelectric immunoassay based on nanoprobe immunoagglutination was proposed for rapid and sensitive detection of IgG. The nanoprobes were prepared by labeling gold nanoparticles with anti-IgG antibody. And a piezoelectric sensor was used to monitor the changes in solution density and viscosity caused by the immunoagglutination of IgG and anti-IgG nanoprobes. The effects of pH and ionic strength were investigated. It is found that the developed immunoagglutination assay is sensitive to IgG with the detection limit of 1.12, 0. 72, 0. 70 nmol/L to the detection time of 10, 20, 30 min, respectively, and it is unnecessary for immobilization of immunoactive entities.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2007年第4期649-651,共3页
Chemical Journal of Chinese Universities
关键词
压电免疫传感
纳米探针
凝聚反应
IGG检测
Piezoelectric immunosensor
Immuno-nanoprobe
Immunoagglutination
IgG detection
