碘对大鼠皮肤创伤修复影响的实验研究

Role of iodine on wounds in the skin of rats

目的:探讨碘对皮肤创伤修复的影响。方法:建立大鼠背部双侧圆形皮肤全层创伤模型,观察创面愈合情况,测量其面积。应用免疫组化SP法检测血管内皮细胞生长因子(VEGF)的表达,用图像分析系统分析其表达情况,用光学显微镜观察组织形态学特征。结果:适当浓度的碘可明显缩短创面的愈合时间。创伤后1-7 d,10HI组大鼠创面的愈合率显著高于生理盐水组(P<0.05或P<0.01),而1 000HI组大鼠创面愈合率则与生理盐水组相当。组织形态学结果显示,在大鼠创伤组织修复早期,10HI能够促进纤维细胞增殖及新生毛细血管的形成,增强成纤维细胞与血管内皮细胞对VEGF的表达,1 000HI则阻碍组织修复。结论:适当浓度的碘有促进大鼠皮肤创伤修复的作用,过高浓度的碘则阻碍伤口愈合。

Objective： To study the effect of iodine on wounds in the skin of rats. Methods： A bilateral round full-thickness wound was made on the back of Wistar rats. The benefit effect of iodine on the skin wound was evaluated by determining closure time and contracting ability of the skin wound. The expression of VEGF in the wound tissues was determined by immunohistochemical techniques and an image analysis system. Results： The closure time of skin wounds was significantly shorter in the 10HI-treated groups than that in the sodium chloride-treated groups. The percentage of the wound contraction was higher in the 10HI-treated groups than that in the sodium chloride-treated groups （ P 〈 0.01 and P 〈 0,05） between the 1st and the 7th day after wounding. There was no difference between the 1 000HI- treated groups and the sodium chloride-treated groups, Histopathological results showed that 10HI could promote the formation of new bom capillaries and the proliferation of fibroblasts at an early period of wound healing and promote the expression of VEGF from fibroblasts and the vascular endothelial cells; whereas 1 000HI reversed these. Conclusion： Iodine of benefit concentration has the ability, to promote healing in the skin of rats, but iodine of high concentration can delay healing.