碱性成纤维细胞生长因子和转化生长因子-β在近视家族胎儿巩膜的表达

Primary study on the expression of bFGF and TGF-β in the posterior sclera of human fetuses with family-based myopia

目的:通过检测相同胎龄父母近视程度不同的胎儿后极部巩膜碱性成纤维细胞生长因子(basicfibroblast growth factor,bFGF)和转化生长因子-β(transforming growth factor-beta,TGF-β)的表达水平,探讨bFGF和TGF-β在人类近视遗传和近视形成过程中是否发挥作用。方法:对16例14周以下引产的胎儿,按父母无近视和父母近视>-4.00D分为对照组和实验组,每组8例,免疫组化和RT-PCR方法检测后极部巩膜bFGF和TGF-β的表达,确定两组是否存在差异。结果:两组bFGF和TGF-β在巩膜中均有表达,实验组BFGF的表达较对照组高,两者比较差异有统计学意义(P<0.05),实验组TGF-β的表达较对照组低,两组比较差异有统计学意义(P<0.05)。结论:近视家族胎儿在巩膜发育阶段就有bFGF和TGF-β的某些改变,bFGF和TGF-β在人类近视的形成和遗传中可能发挥一定作用,是近视多基因遗传因素中的相关因素之一。

Objective： To evaluate the expression of basic fibroblast growth factor（bFGF） and transforming growth factor-beta（TGF-β） of the posterior sclera in human fetuses of the same month. Methods： Sixteen eyes of human 14-week fetus were divided into two groups： the experimental group whose parents were myopia with a refraction over - 4.00 D and the control group whose parents were without myopia. The expressions of bFGF and TGF-β in the posterior sclera were determined and quantitatively analyzed by immunohistochemistry and RT-PCR. Results： The expression of bFGF in the experimental group was significantly higher than that in the control group （ P 〈 0.05）, while on the contraiy, the expression of TGF-β in the experimental group was significantly lower than that in the control group （P 〈 0.05）. Conclusion： The expressions of bFGF and TGF-β in the posterior sclera of the fetal eyes of the same fetal month are significantly different between myopia families and non-myopia families.