Notch-1调控视网膜前体细胞向视网膜神经节细胞分化

Regulating effect of Notch-1 on retinal progenitor cells differentiating into retinal ganglion cells

目的研究Notch-1对视网膜前体细胞（RPC）向视网膜神经节细胞（RGC）分化的调控作用。方法分离培养胚胎14d龄Sprague-Dawley大鼠的RPC，实验组和对照组分别用含有Notch-1反义寡核苷酸链和无关序列寡核苷酸链的培养液进行诱导分化14d，倒置相差显微镜每天观察细胞的生长和分化情况，Thy1.1标记RGC并进行计数。结果实验组和对照组的RPC都能分化为多种视网膜细胞类型，包括Thy1.1阳性的RGC，但两组RPC向RGC分化的百分比不同。实验组和对照组RGC的百分比分别为（16.57±4.31）%和（31.19±6.90）%，两组比较差异有统计学意义（t=9.84，P〈0.001）。结论Notch-1对RPC的分化具有负向调控作用，阻断Notch-1能促进RPC向RGC分化。

Objective To investigate the regulating effect of Notch-1 on retinal progenitor cells （RPC） differentiating into retinal ganglion cells （RGC）. Methods RPC of 14-day embryonic SD rats were induced and differentiated in the culture medium with Notch-1 antisense oligonucleotides （experimental group） or missense oligonucleotides （control group） for 14 days. The condition of growth and differentiation of the cells were observed daily under the phase-contrast microscope. RGC were identified by Thyl. 1 immunocytochemistry methods and the cellular number was counted. Results RPC in both of the two groups differentiated into various retinal cells, including Thyl. 1-positive RGC. The percentage of RGC derived from RPC was 31.19%±6.90% in experimental group and 16.57%±4.31% in control group, and the difference was statistically significant （t = 9.84, P〈0. 001）. Conclusion Notch-1 may down-regulate the differentiation of RPC, and the inhibition of Notch-1 may promote RPC differentiating into RGC.