缬沙坦对肾血管性高血压大鼠心肌肥厚及结缔组织生长因子表达的影响

Effects of valsartan on ventricular hypertrophy and expression of connective tissue growth factor in the rats with renovascular hypertension

目的:研究肾血管性高血压(RVH)大鼠心肌肥厚及结缔组织生长因子(CTGF)的表达情况,探讨缬沙坦逆转左室重构的可能机制。方法:用两肾一夹方法构建RVH大鼠模型。以左心室重量(LVW)、LVW与体重比值(LVW/BW)作为心肌肥厚的指标,分别应用RT-PCR法和免疫组织化学染色法检测CTGF的mRNA和蛋白质的表达。结果:①RVH大鼠术后第8周出现心肌肥厚,第12周进一步加重。缬沙坦治疗后,心肌肥厚明显减轻(P<0.01)。②RVH未治疗组CTGF mRNA及蛋白表达与假手术组比较显著增加;缬沙坦治疗4或8周后,其表达较相应未治疗组显著降低(P<0.05)。结论:RVH大鼠CTGF表达增多,缬沙坦能部分抑制其过度表达。这可能是AT受体阻滞剂预防或逆转心肌肥厚重构的机制之一。

Objective：To study effects of valsartan on ventricular hypertrophy and expression of connective tissue growth factor in the rats with renovascular hypertension （RVH）. Method： One group of two-kidney one-clip （2K1C） RVH rats received valsartan （30 mg · kg^-1 · d^-1） and the other group with no treatment for 4 or 8 weeks. The left ventricular weight（LVW） and the ratio of left ventricle weight to body weight（LVW/BW） were measured. The CTGF mRNA and protein in the left ventricular myocardium were assayed by semi-quantitative RT-PCR and immunohistochemical methods. Result： The expression of CTGF mRNA and protein in the left ventricular myocardium increased significantly in 1RVH rats without treatment but decreased significantly after 4wks or 8wks treatment with valsartan（P〈0. 05）. Conclusion：Increased expression of CTGF mRNA and protein in left ventricular myocardium of rats with renovascular hypertension can be partially prevented by valsartan. This may be one of the mechanisms for AT1 receptor antagonists to prevent and reverse left ventricle remodeling.