人脑标本不同区域多巴胺转运体分布的免疫放射自显影研究

Immunoautoradiographic studies on the distribution of dopamine transporter in diffrerent regions of postmortem human brain

目的定量分析人脑标本不同区域多巴胺转运体(DAT)的免疫反应强度,为神经影像检查DAT变化的参照区选择提供依据。方法取自8例男性尸脑标本的脑组织块经石蜡包埋切片后,以免疫放射自显影显示黑质、壳及尾状核、扣带回、额叶、枕叶和小脑皮质的DAT免疫反应强度。结果所观察的脑区中,DAT免疫反应强标记主要分布在黑质、尾状核及壳,小脑皮质标记最弱。定量分析发现,小脑皮质的DAT免疫反应强度分别为额叶、扣带回及枕叶的1/3.50、1/3.72、1/1.28,仅为黑质、尾状核、壳的1/8.33、1/11.67、1/8.56。结论小脑皮质在所检脑区中DAT含量最低,可作为神经影像检查DAT变化的参照区。

Objective Immunoreactive intensity of dopamine transporter （DAT） was quantitatively analyzed in the different regions of postmortem human brain in order to provide evidence in selecting an appropriate reference region for neuroimaging in measurement of the altered DAT. Methods The brain tissue blocks taken from 8 male cadavers were paraffin-embedded and sectioned,and immunoautoradiography was used to display the difference of DAT immunoreactive intensity in the substantia nigra, putamen and caudate nucleus, cingulate cortex, frontal cortex, occipital cortex and cerebellar cortex. Rcsults The highest DAT density was found mainly in the substantia nigra, caudate nucleus and putamen, while the lowest density was only seen in cerebellar cortex. Quantitative analysis revealed that the intensity of DAT immunoreactivity （DAT-IR） in cerebellar cortex was respectively 1/3. 50, 1/3. 72, 1/1.28 of that in frontal cortex, cingulate cortex and occipital cortex, and it was only 1/8. 33, 1/11. 67, 1/8. 56 that of substantia nigra, caudate nucleus and putamen. Conclusions The cerebellar cortex has the lowest DAT as compared to other examined brain regions, and it can be used for a reference region in neuro- imaging to detect altered DAT.