四肢火器伤受紫外线照射后碱性成纤维细胞生长因子的表达

Effect of ultraviolet C irradiation on the expression of basic fibroblast growth factor in gunshot wounds in limbs

目的研究四肢火器伤受不同剂量短波紫外线(UVC)照射后伤口肉芽组织中碱性成纤维细胞生长因子(bFGF)表达变化。方法建立兔四肢软组织火器伤模型,UVC30mJ/cm2和60mJ/cm2分别进行伤道内照射。采用免疫组化和原位杂交的方法观察组织中bFGF的表达。结果致伤照射后7～21天,60mJ/cm2照射组和30mJ/cm2照射组bFGF在mRNA水平与蛋白水平的表达均高于对照组(P<0.05),以60mJ/cm2照射组更为显著;照射后21天,60mJ/cm2照射组bFGF的mRNA水平显著降低,低于30mJ/cm2照射组(P<0.05)。结论在伤口愈合早期UVC照射能促进bFGF的表达,以60mJ/cm2的作用更显著。但不同剂量UVC促进bFGF表达的时相性有所不同。

Objective To investigate the effect of different irradiation doses of ultraviolet C ray （UVC） on the expression of basic fibroblast growth factor （bFGF） in granulation tissue of gtmshot wound in rabbit limbs, Methods Alter giving 30mJ/cm^2 or 60mJ/cm^2 UVC to the gunshot wound of soft tissue of limbs, the expression of bFGF was observed both at the mRNA level and the protein level by the methods of in situ hybridization and immunohistochemistry, respectively. Results On the 7th day after UVC irradiation, the expression of bFGF in UVC treated groups was significantly higher than that in control group （P〈0. 05） at both the mRNA and the protein levels, especially in the 60mJ/cm^2 group. While on the day 14 after UVC treatment, the bFGF protein and mRNA levels in 60mJ/cm^2 group de creased rapidly and were lower than that in the other two groups （P〈0.05）. Conclusion In the initial stage of wound healing, the treatment of UVC irradiation promoted the expression of bFGF at different dose levels, but the time phases of the promotion effect of different doses were diverse